By Kathleen M. Roberts
On September 8, 2017, the U.S. Department of Energy (DOE) selected an additional four Productivity Enhanced Algae and Toolkits (PEAK) projects to receive up to $8.8 million. The projects aim to develop high-impact tools and techniques that will increase the productivity of algae organisms to reduce the costs of producing algal biofuels and bioproducts. In total, DOE has awarded over $16 million in funding to the initiative.
The project winners include:
- Colorado School of Mines, in partnership with Global Algae Innovations, Pacific Northwest National Laboratory, and Colorado State University, which will use advanced directed evolution approaches in combination with high-performance, custom-built, solar simulation bioreactors to improve the productivity of robust wild algal strains;
- University of California, San Diego, which will work with Triton Health and Nutrition, Algenesis Materials, and Global Algae Innovations on the development of genetic tools, high-throughput screening methods, and breeding strategies for green algae and cyanobacteria, targeting robust production strains;
- University of Toledo, in partnership with Montana State University and the University of North Carolina, which will cultivate microalgae in high-salinity and high-alkalinity media to achieve productivities without needing to add concentrated carbon dioxide, and deliver molecular toolkits, including metabolic modeling combined with targeted genome editing; and
- Lawrence Livermore National Laboratory, which will ecologically engineer algae to encourage growth of bacteria that efficiently remineralize dissolved organic matter to improve carbon dioxide uptake and simultaneously remove excess oxygen.
By Lauren M. Graham, Ph.D.
Sandia National Laboratories (Sandia) is investigating whether algae can be used to transform the Salton Sea, one of California’s largest and most polluted lakes, into a productive and profitable resource. The Salton Sea Biomass Remediation project (SABRE), which is funded by the U.S. Department of Energy’s (DOE) Bioenergy Technologies Office (BETO), aims to use algae to rid the lake of pollutants while creating a renewable, domestic source of fuel and other chemicals. Algae are known to thrive in environments like the Salton Sea, which contains elevated levels of nitrogen and phosphorus due to agricultural runoff.
In the first phase of the project, Sandia partnered with Texas A&M AgriLife Research to investigate the efficacy of a new algal farming method, known as the “Algal Turf Scrubber” floway system. The algae consume the nitrogen and phosphorus from the polluted water that is pumped into the system using solar-powered pumps. Clean water is then deposited back into the lake.
The second phase began in May and the initial results indicate that the system can produce a quantity of algae comparable to raceways, the traditional algal farming method. The algae being grown are native to the area which makes it more resistant to attacks from local pathogens and predators. By helping to clean polluted water, Sandia researchers have overcome a major criticism of algae as a biofuel source, specifically that farming algae requires too much water. Additionally, the removal of pollutants, such as nitrogen, phosphorus, and other fertilizer components, is expected to provide a model of remediation for algae blooms.
By Lauren M. Graham, Ph.D.
On July 11, 2017, DOE announced the selection of three projects focused on reducing the costs of producing algal biofuels and bioproducts that will receive up to $8 million in funding. The projects aim to generate high-impact tools and techniques for increasing the productivity of algae organisms and cultures and biology-focused breakthroughs. The project winners include:
- Lumen Bioscience, which will work with the National Renewable Energy Laboratory on the agricultural production of algae on otherwise non-productive land in rural eastern Washington State by rapidly engineering strains that grow robustly in seawater, resist contamination and predation, and accumulate substantial amounts of energy-rich components;
- Global Algae Innovations, which will work in partnership with Sandia National Laboratories, University of California at San Diego – Scripps Institution of Oceanography, and the J. Craig Venter Institute to deliver a tool for low cost, rapid analysis of pond microbiota, gather data on the impacts of pond ecology, and develop new cultivation methods that utilize this information to achieve greater algal productivity; and
- Los Alamos National Laboratory, which will work with Sapphire Energy to help the algal research and development community better understand these metrics at commercial scales by evaluating rationally designed pond cultures containing multiple species of algae, as well as beneficial bacteria, to achieve consistent biomass composition and high productivity.
By Lauren M. Graham, Ph.D.
On June 19, 2017, Synthetic Genomics Inc. announced a breakthrough in its collaboration with ExxonMobil involving the modification of an algae strain that more than doubled its oil content to 40 percent without significantly inhibiting the strain’s growth. Synthetic Genomics researchers identified a genetic switch that could be fine-tuned to regulate the conversion of carbon to oil in the algae species, Nannochloropsis gaditana, and established a proof-of-concept approach for the new process. The achievement is a key milestone in the partnership that aims to demonstrate that algae can be incredibly productive as a renewable energy source with a corresponding positive contribution to our environment. Additional research, testing, and analysis is required to ensure the process is commercially viable.
By Lynn L. Bergeson
Researchers at the University of California San Diego (UCSD) and Sapphire Energy completed the first EPA-sanctioned outdoor field trial for GE algae, which was focused on understanding how GE algae perform in outdoor cultivation. The 50-day experiment studied algae (Acutodesmus dimorphus) that was GE with genes for enhanced fatty acid biosynthesis and recombinant green fluorescence protein (GFP) expression under real world conditions in parallel with non-GE algae strains. The results demonstrate that GE algae can be cultivated outdoors while maintaining the GE traits, and that the specific GE algae investigated does not adversely impact native algae populations. According to the researchers, the study provides a framework to evaluate GE algae risks associated with outdoor GE algae production, which offers the promise of producing sustainable food, fuel, and other valuable products.